Bioconjugate & ADC Immunogenicity Assays

The new EpiScreen® 2.0 time course T cell assay is used to assess the potential immunogenicity of therapeutic antibodies and other proteins before they reach the clinic. It is sensitive, specific, data-rich, MoA reflective, customizable and repeatable.

The new EpiScreen® 2.0 DC:T cell assay is used to assess the potential immunogenicity of therapeutic antibodies or other proteins that directly modulate CD4+ T cell activation.

EpiScreen® 2.0 Cytokine Screen™ evaluates the potential of therapeutic proteins to activate innate and adaptive immune responses resulting in rapid and excessive cytokine release, which can produce significant toxicity. Abzena have developed an enhanced in vitro assay, Cytokine Screen™, to evaluate the risk of biopharmaceutical products causing cytokine release syndrome prior to clinical trials. Available as whole blood to assess innate responses or PBMC format to study adaptive responses with standard or bespoke cytokine panels.

EpiScreen® 2.0 T cell epitope mapping identifies the precise number and location of T cell epitopes within an antibody or protein. These can then be eliminated by generating humanized antibodies or deimmunized proteins.

EpiScreen® 2.0 MAPPs detects and sequences naturally processed and presented peptides in the context of MHC Class II utilizing highly powerful mass spectrometry.

iTope-AI and T Cell Epitope Database (TCED™) are in silico tools to rapidly screen antibodies and proteins for potential immunogenicity. Combined, they provide a more accurate prediction of T cell epitopes than other in silico technologies that rely on MHC class II binding analysis alone.

Abzena brings together expertise in immunology with the technology required to perform bespoke immunology assays. Our large bank of characterized PBMC, regulated by the human tissue authority, supplies fresh whole blood, buffy coats, apheresis cones and leukopaks as well as a large range of cell lines that can be used for collaborative studies. Typical read-outs include immunophenotyping, activation markers, cytotoxicity, live cell imaging and cytokine analysis by Luminex and FluoroSpot.