Complex Biologics CDMO+ CRO - Abzena

Custom Immunology Assays

Our experts have over 20 years of experience in immunoassay development. Start smarter, with Abzena.

Custom immunology assays can be set up using a choice of test system, stimulant and readout. Contact our team of experts to discuss options for your program.

Abzena partners with you to deliver custom assay development and immunology solutions, combining technical expertise with a broad range of validated technologies.

Our large bank of characterized PBMCs, regulated by the Human Tissue Authority, supports immunogenicity assay development and in vitro immunogenicity assays. We provide fresh whole blood, buffy coats, apheresis cones, leukopaks, and a wide selection of cell lines for collaborative studies. Cross-group projects are particularly effective in advancing reliable results.

Abzena’s expertise spans the full scope of biologic assay development, working seamlessly across departments to accelerate preclinical programs and ensure consistent data quality.

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Examples of recent custom assay development and technology projects include:

  • Functional studies using primary dendritic cells to evaluate complex novel therapeutics.
  • Assessment of B cell functionality following CD40 ligand activation.
  • Binding, ADCC, and apoptosis assays using isolated eosinophils.
  • Viral epitope identification using MAPPs, in silico prediction, CD8+ DC:T cell assays, and FluoroSpot.
  • Functional assays for bispecific antibodies supporting early immunogenicity assays.
  • T cell-specific cytotoxicity assays for bispecific antibodies using live-cell imaging.
  • Evaluation of drug candidate effects on dendritic cell maturation and migration.
  • Neutrophil activation studies, including migration assays and ROS/MPO release analysis.

Types of cells:

  • Primary dendritic cells
  • CD25+ depleted CD4+ T cells
  • CD8+ T cells
  • B cells
  • Eosinophils
  • NK cells
  • PBMC
  • Neutrophils
  • T and B cell lines
  • Extensive selection of cell lines at Abzena

Modalities tested:

  • Antibody-drug conjugates
  • Bi-specifics, tri-specifics
  • Cytokine fusions
  • Cytokine receptor Antagonists
  • Checkpoint inhibitors
  • Chemokine Receptor
  • Enzymes
  • Fragments
  • Nanobodies (VHH)
  • Signaling receptor inhibitors
  • TLR agonists
  • Viral proteins
  • Therapeutic peptides
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Custom Immunology Assays FAQs

What is custom assay development?

Custom assay development tailors analytical or functional assays to a specific molecule or program. Abzena designs assays that deliver accurate, reproducible data aligned with your study goals.

What does the assay development process involve?

The assay development process includes design, optimization, validation, and data analysis. Abzena ensures each step meets regulatory and scientific requirements for reliable results.

How do custom immunology assays support drug development?

Custom immunology assays help evaluate immune response, mechanism of action, and safety. They guide decisions during preclinical testing and immunogenicity assay development.

Does Abzena offer in vitro immunogenicity assays?

Yes. Abzena performs in vitro immunogenicity assays using human PBMCs, dendritic cells, and T cells to assess immune activation and predict clinical relevance.

Can Abzena integrate custom assay development into broader programs?

Yes. Our assay development experts collaborate across discovery, process development, and immunogenicity assays to create cohesive data packages supporting biologics development.

Related Resources & Insights

Immunogenicity Assays

An integral service to biologic drug development. Immunogenicity assays help you better understand your drug earlier, allowing you to start smart, and finish fast.

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TCED™ & iTope-AI – A powerful tool for in silico immunogenicity assays

A powerful tool for in silico immunogenicity assays and design of deimmunized proteins & antibodies.

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Blog: Immunogenicity Assessments – Frequently Asked Questions (FAQs)

Discover our blog of frequently asked questions on immunogenicity assays and addressing the complexities of immunogenicity in biologic therapies.

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EpiScreen® 2.0 DC:T Cell Assay

Assessing antibody immunogenicity and the 'adjuvant effect' of different product formulations on immunogenicity.

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EpiScreen® 2.0 MAPPs: MHC Class II Associated Peptide Proteomics

Identifying MHC Class II bound peptides processed and presented by dendritic cells (DCs) from therapeutic proteins/mAbs.

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EpiScreen® 2.0 T Cell Epitope Mapping

Identifying CD4+ T cell epitopes within protein sequences in order to design deimmunized variants.

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EpiScreen® 2.0 Time Course T Cell Assay

These assays have been used extensively as a standard for preclinical immunogenicity testing.

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Episcreen® 2.0 Cytokine Screen™

We can assess a biopharmaceutical product's immune modulating potential by assessing its ability to stimulate cytokine production.

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