EpiScreen® 2.0 DC:T Cell Assay

The EpiScreen® 2.0 DC:T cell assay is used to assess the potential immunogenicity of antibodies and proteins that directly modulate T cell activation. Assays are performed using dendritic cells (DC) derived from peripheral blood mononuclear cells (PBMCs), typically from 50 individual donors, with a distribution of HLA-DR allotypes (coverage and frequency) representing the human population of interest.

The isolated monocytes are differentiated in vitro to an immature DC phenotype. These cells are then loaded with the test protein before inducing further differentiation to a mature DC phenotype.

Once matured, the cells are incubated with autologous CD4+ T cells before measuring T cell proliferation using EdU incorporation and T cell activation by assessing CD25/OX-40 upregulation.

 

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Optional cytokine profiling and additional immunophenotyping are also available.

Pre-screen tests using a small numbers of donors can be performed to evaluate if there is direct modulation of T cell activation prior to choosing a time course or DC:T cell assay.

Our immunogenicity assay services are tailored for each project to ensure that the objectives are met or exceeded. Experienced project teams are assigned to each study focusing on progressing projects through to results, minimizing risk.

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