Antibody-Dependent Cellular Phagocytosis (ADCP) Assays
ADCP is a potent mechanism of elimination of antibody-coated foreign particles such microbes or tumour cells. Engagement of FcγRIIa and FcγRI expressed on macrophages triggers a signalling cascade leading to the engulfment of the IgG-opsonised particle.
In our ADCP assay, the antigen-expressing cancer cells are incubated in the presence of a titration of mAb and the human leukaemia monocytic cell line THP-1. Both effector and target cells are fluorescently labelled and cell engulfment is measured by flow cytometry. Phagocytosis can also be confirmed using microscopy or imaging cytometry.
Working with Abzena
Abzena undertakes bioassays on behalf of clients as standalone projects or as part of larger development projects. We focus on developing the correct bioassay to help you understand your molecule.
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Rituximab induces Fc-mediated phagocytosis of Ramos cells by the monocytic cell line THP-1. Engulfment of Ramos cells is confirmed by fluorescence microscopy.