CHO Cell Line Performance with 2G UNic® Technology Case Study

To assess the impact of 2G UNic® vector technology on protein expression in CHO cell lines, this real-world case study from Abzena compares standard expression vectors with 2G UNic® optimized constructs across multiple CHO host backgrounds.

Using a real-world case study, we demonstrate how well-optimized random integration methods can produce comparable – or better – titers, productivity, and stability. By focusing on proper vector design, host cell line selection, and robust screening strategies, random integration can yield high-performing clones efficiently.

This case study was taken from our recent comparison of random integration (as offered by 2G UNic® technology) with semi-targeted transposase-based systems. We looked at their respective advantages and limitations in a whitepaper entitled, ‘Random Integration vs. Semi-Targeted Integration in Stable Cell Line Development’. Request your copy of the case study to find out Abzena’s findings.