Protein & Antibody Expression

Transient expression involves the temporary introduction of genetic material into the host cells. There is no permanent integration into the host genome, and so no selection is required. Consequently, it is the faster of the two approaches, allowing researchers to generate recombinant proteins in days rather than weeks. It’s an ideal choice for early research, small-scale screening, or the generation of material for initial analytical or structural needs.

Transient protein expression in CHO cells offers several advantages in the context of monoclonal antibody production:

• Speed: Proteins can be produced within a few days of transfection.
• Flexibility: Excellent for evaluating multiple constructs or formats in parallel, or for examining, for example, the effect of different chain ratios for a complex bispecific.
• Scale: Best suited to small-scale production (milligram to gram quantities).
• Cost: Transients are generally more cost-effective than stables.

Transient expression systems are particularly beneficial when evaluating multiple candidates or on an accelerated discovery timeline. At Abzena, we offer a range of rapid transient production systems, including ExpiCHO™, enabling clients to test design concepts quickly before committing to more extensive GMP-ready cell line development.

Abzena offer an optimized ExpiCHO™ transient system allows for the generation of up to ~500 mg of recombinant antibodies, whilst our CHO-K1 stable pools allow for larger-scale protein production up to 5-10g scale in as little as 8 weeks.

All our proteins and antibodies undergo rigorous quality control, which includes the assessment of purity and integrity.

Extended analytics are also available to further evaluate the standard and novel characteristics of your biologic.

In addition to standard methods, we also have significant experience in developing custom purification strategies for non-standard proteins using our purification suite, and when coupled with our analytics & bioassays team, we can provide a complete package of protein production and characterization.