Binding to Fc-gamma and neonatal Fc receptors
The Fc-gamma receptor family includes 5 members: FcγRI (CD64), FcγRIIa (CD32a), FcγRIIb (CD32b), FcγRIIIa (CD16a) and FcγRIIIb (CD16b), which differ in their affinities for the different IgG subclasses. Fc-gamma receptors are expressed on a number of immune cells and by interacting with these receptors, antibodies can regulate immune responses.The neonatal Fc receptor (FcRn) is expressed in different organs where its functions can vary. Interactions between FcRn and the Fc portion of an IgG have been linked to antibody catabolism, transport and potential tissue-specific toxicities. FcRn is best known for its ability to protect antibodies from degradation, therefore improving their half-life in circulation.
Human IgG receptors: (Pierre Bruhns Blood 2012;119:5640-5649). ©2012 by American Society of Hematology
Kinetics of antibody or ADC binding to FcγRI, FcγRIIaH131, FcγRIIaR131, FcγRIIb, FcγRIIIaV158, FcγRIIIaF158, FcγRIIIb and FcRn can be assessed at Abzena using our Biacore T200.
Alternatively, commercially available cell lines transfected with selected Fc-gamma receptors can be used to assess antibody or ADC binding by flow cytometry. At the interface between binding and functional assays, reporter cell lines expressing Fc-gamma receptors are also an option offered at Abzena.
Working with Abzena
Abzena’s undertakes bioassays on behalf of clients as standalone projects or as part of larger development projects. We focus on developing the correct bioassay to help you understand your molecule and will help guide you in the correct.
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