Characterisation of Drug Resistance
Some drug resistant cell lines are commercially available but more relevant MDR models can also be generated at Abzena using your cell line and compound of choice.
Typically, drug resistant cell pools are characterised at regular intervals for resistance to the compound in viability assays. Expression of MDR markers is also monitored alongside retention of Pgp substrates in the modified cells.
Characterisation of Parental and Docetaxel resistant prostate cancer cell line: Expression of three MDR markers was shown to be upregulated on the resistant cell line by flow cytometry. The anti-proliferative effect of Docetaxel was also reduced on the resistant cell line when compared to the parental line.
Working with Abzena
Abzena’s undertakes bioassays on behalf of clients as standalone projects or as part of larger development projects. We focus on developing the correct bioassay to help you understand your molecule and will help guide you in the correct.
To get more information, a quote or to schedule a teleconference please contact us.