Cell Viability Assays | Cytotoxicity Assays | Abzena

Viability & Cytotoxicity Assays

Cell viability and cytotoxicity assays are the go-to assays for assessing the potency of antibody drug conjugates (ADCs) and cytotoxic payloads.

They are amenable for high throughput screening of compounds as well as characterisation of lead candidates. They are also routinely used as drug substance release assays for ADCs.

We offer a standard 2D cell viability assay that can easily be applied to your cell lines of interest. A range of assay variations is also available and each assay can be customised to match your specific requirements.

Viability & cytotoxicity assay options for ADC and payload characterisation:

Standard 2D cell viability assay

To assess the anti-proliferative effect (cytotoxic and/or cytostatic) of compounds. Cancer cell lines are incubated in the presence of an 8-point titration of compounds in triplicate. After 4 days in culture, the ATP level is measured as an indicator of cell viability.

Limited exposure cell viability assay

To assess the anti-proliferative effect of ADCs after a single/a few cycle(s) of internalisation. After a short incubation period, the unbound ADC is removed and cells allowed to proliferate for 4 days.

Competition cell viability assay

To confirm that the potency of an ADC is target-mediated by co-incubating the cancer cell lines in the presence of a fixed concentration of ADC and a titration of mAb.

Stress test cell viability assay

To assess ADC stability in plasma and its effect on in vitro potency by pre-incubating the ADCs in plasma prior performing a standard 2D cell viability assay.

Multidrug resistance cell viability assay

To assess in parallel the anti-proliferative effect of compounds on multidrug resistant and control cancer cell lines.

3D cell viability assays

To assess tumour penetrability and anti-proliferative effect of compounds in an in vitro system mimicking tumour morphology. Spheroid formation is established under a set of culture conditions before assessment of the compounds.

Cell cytotoxicity assay

Cancer cell lines are incubated in the presence of an 8-point titration of compounds in triplicate. After 4 days in culture, dead-cell protease activity, which is released from cells that have lost membrane integrity, is measured.

Working with Abzena

Abzena undertakes bioassays on behalf of clients as standalone projects or as part of larger development projects. We focus on developing the correct bioassay to help you understand your molecule.

To get more information, a quote or to schedule a teleconference please contact us.


SK-BR-3  2D Viability and Cytotoxicity Assays using Her2 expressing SK-BR-3 cell line as target
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